THESIS ON SCRUB TYPHUS

J Assoc Physicians India. Recently, in India there are scrub typhus reports from Goa where the disease was not documented earlier indicating the occurrence of the disease in previously unidentified regions [ 3 ]. The diagnosis of scrub typhus is generally made by the history and clinical presentation. The Immunofluorescence antibody IFA test has its limitations in terms of cost and expertise required. Expert Rev Anti Infect Ther. However, it lacks specificity and sensitivity [ 15 ].

J Assoc Physicians India. Genome-based construction of the metabolic pathways of Orientia tsutsugamushi and comparative analysis within the Rickettsiales order. This again emphasises the fact that Weil-Felix test is not a sensitive test for diagnosis of scrub typhus. The test was standardised with serum samples from healthy blood donors. Recently, in India there are scrub typhus reports from Goa where the disease was not documented earlier indicating the occurrence of the disease in previously unidentified regions [ 3 ]. Clinical diagnosis of scrub typhus is challenging as the signs and symptoms of scrub typhus are similar to other febrile illnesses. The name scrub typhus is derived from the prevalence of the mites in areas of heavy scrub vegetation.

Am J Trop Med Hyg. A preliminary report from Pondicherry.

Scrub typhus in Darjeeling, India: Expert Rev Anti Infect Ther. Indian J Med Microbiol. The study was conducted only after obtaining written informed consent from the patients.

thesis on scrub typhus

Indian J Med Res. A set of positive and negative controls were included along with every test reaction.

thesis on scrub typhus

Evaluation of tests for serological diagnosis of scrub typhus. The organism is also extremely theesis requiring BSL—III facility for its culture which is impractical in most laboratories in India [ 15 ].

  DISSERTATION PAYANTE DEVOIRS

J Clin Diagn Res. Find articles by K. Conclusion The medical fraternity needs to understand the pitfalls of currently available Weil-Felix test which is still common in India and must include tests with better performance like IgM ELISA and nested PCR in the diagnostic algorithm of scrub typhus to reduce the burden of the disease.

Diagnosis of scrub typhus. None Acknowledgement for financial support and specific contribution only: Therefore the disease has been urbanised and the prevalence of the disease has broadened further [ 1 ].

Scrub typhus in patients reporting with acute febrile illness at a tertiary health care institution in Goa. The most widely performed laboratory test in India is Weil-Felix test which has low sensitivity and specificity. Molecular tests like nested PCR for diagnosis acts as a viable, early and accurate diagnostic tool as compared with that of serological tests. Is scrub typhus a cause of febrile illness among paediatric population of delhi?

Serodiagnosis of Scrub Typhus at a Tertiary Care Hospital from Southern India

Published online Nov 1. Please review our privacy policy. In this study an attempt has been made to know the seropositivity in clinically suspected scrub typhus patients.

First case of scrub typhus with meningoencephalitis from Kerala: Therefore it is recommended to perform both pathogen and antibody based tests. Results After screening patients with acute febrile illness with a battery of diagnostic tests such as Para F for malaria, Widal tests, ICT for dengue and Ig M ELISA for leptospirosis, 63 samples reacted positively for above mentioned tests and were excluded.

  CMC THESIS ISKWIKI

The infection is transmitted by bite of larval stage chiggers of mites belonging to the family Trombiculidae. The mite acts as both vector and reservoir of the bacteria and efficiently transmits to its off springs through transovarian transmission.

InKim et al. The culturing of the organism is not only inappropriate as the time duration of the same is not effective enough for the early diagnosis of scrub typhus. High-level expression of a kilodalton protein gene bor56 of Rickettsia tsutsugamushi Boryong and its application to enzyme-linked immunosorbent assays. A study was conducted in Delhi among the febrile paediatric patients which did not have definitive diagnosis.

Serodiagnosis of Scrub Typhus at a Tertiary Care Hospital from Southern India

IFA being the gold standard for serology of scrub typhus is considered as imperfect with its own limitations [ 15 ]. The current gold standard for serology is Indirect Immunofluorescence Antibody IFA test which uses a fluorescence labelled anti-human immunoglobulin to detect antibodies in patients serum that have bound to immobilised bacterial antigen that is coated on the IFA slide.

Int J Microbiol Res Rev. The patients attending this hospital as tthesis inpatients and outpatients who were clinically diagnosed to have typhus fever were included.